Utilizing single-cell mass spectrometry to review antimicrobial remedy failure


Laura-Isobel McCall, Ph.D., and Zhibo Yang, Ph.D., co-principal investigators and affiliate professors of chemistry and biochemistry within the Dodge Household School of Arts and Sciences on the College of Oklahoma, have been awarded a prestigious R01 grant from the Nationwide Institutes of Well being for his or her venture, “Novel single-cell mass spectrometry to evaluate the position of intracellular drug focus and metabolism in antimicrobial remedy failure.”

Our venture builds upon Dr. Yang’s analysis that exhibits variations in chemotherapy medicine inside particular person most cancers cells. Utilizing his methods with single-cell mass spectrometry, if we discover that cells with persistent pathogens even have much less intracellular drug ranges, then we might clarify antimicrobial remedy failure.”

Laura-Isobel McCall, Ph.D., affiliate professor of chemistry and biochemistry within the Dodge Household School of Arts and Sciences, College of Oklahoma

McCall’s lab, which works on parasitic infections, will collaborate with Carolyn Ibberson, Ph.D., assistant professor of microbiology within the Dodge Household School of Arts and Sciences, and James Papin, Ph.D., affiliate professor of pathology within the OU School of Medication. Ibberson’s lab will give attention to bacterial infections whereas Papin’s will study viral infections and give attention to PAXLOVID, a COVID-19 remedy.

“My lab focuses on mass spectrometry, which is a really delicate method used to detect and establish molecules utilizing hint quantities of samples,” Yang stated. “With this instrument, we will study particular person cells which can be lower than 10 micrometers in dimension to find out their resistance to medicine used to struggle parasites, micro organism and viruses.”

This Nationwide Institutes of Well being grant is anticipated to supply $2.65 million in funding over 5 years.

“I believe this funding acknowledges how thrilling our thought is, and what a big effect our venture can have,” McCall stated. “It additionally acknowledges that our strategy is a brand new mind-set about infectious illness remedy failures. If our speculation is appropriate, it might change how we handle and deal with infections and what we do about antimicrobial remedy failure.”